This paper mainly aims at wind speed calibration by standard wind speed measurements and its influence factor analysis. First, we find out the main influence factors, and then eliminate them, thus make the calibration results more real and reliable. The calculation formula in JJG431 86 verification regulation is used for argument analysis, and the examples are used for verification. The results show that laboratory temperature is the great factor of influence on the standard wind speed, thus it must be temperature controlling. In the calibration of an anemometer, the temperature change of the laboratory should not be more than 3℃. The air density correction coefficient in the table is too sketchy, and the influence values on standard wind speed can not be ignored, therefore we should use the formula to calculate directly and eliminate errors.