Based on the daily meteorological data observed by 37 stations in Jiangsu Province from 1980 to 2009, the spatiotemporal characteristics of comfort index of human body (CIHB) were studied by use of the linear trend and path analysis. The results indicated that the average annual CIHB index in all Jiangsu and the three partitions (the north of the Huaihe River, the area between Yangtze and Huaihe Rivers and the south of Yangtze River) presents a remarkable increasing trend during the past 30 years, and the linear increasing trend of CIHB in Jiangsu is 0.11/a. Except in summer, the average annual CIHB index of Jiangsu in the other three seasons has an obvious ascendant trend. Increasing is the most prominent in spring and the interannual variation is the most remarkable in winter. In addition, the northsouth difference of CIHB index in autumn and winter is more than that in spring and summer. There is a significant positive(negative) correlation between CIHB and temperature (relative humidity and wind speed), furthermore, the temperature has the biggest impact on CIHB. Although the whole summer CIHB index over the past three decades has no obvious change trend, there is still weak rising trend in July and September since the 1990s. Under the background of the global warming, the research of the characteristics of CIHB climate change may provide scientific basis for people taking effective prevention measures for the variation of meteorological elements and meteorological services about tourism and energy industry.