Application of static closed chamber/GC technique to studying fluxes of CO2 and CH4 of terrestrial ecosystems can provide the necessary basis for exploring the sources and sinks of greenhouse gases in the atmosphere. Therefore, using the consistent analysis-quality control-calibration procedures and methods between the flux observation system and the atmospheric background concentration observation system is crucial. In the research, firstly, the air bags storing samples were detected and deled, including the structure analysis of airbags, testing the influence of airbags on the concentrations of CO2 and CH4 storing in the airbags in a certain period of time etc. In accordance with the testing results, some adjustments were applied in the sampling and transportation to minimize pollution of the samples; Secondly, rigorous procedures and methods that were consistent with that of analyzing and correcting the concentration of atmospheric background greenhouse gases were used to analyze the samples, ensuring the data obtained by using the two different observing methods could be accuracy compared; Finally, the optimized processes and methods in this study have been applied to greenhouse gase flux observing experiment on grazed grassland and fenced grassland at Mt.Waliguan during July 2007-July 2008. Highquality observing data of fluxes of CO2 and CH4 were obtained and a preliminary analysis of CO2 and CH4 fluxes was carried out.