The Round-trip Drifting Sounding System (RDSS) can realize vertical detection of the atmospheric temperature profile from ground to the lower stratosphere and the continuous 4h horizontal temperature distribution in the lower stratosphere. Round-trip Drifting Sounding observation tests have been carried out in the middle and lower reaches of the Yangtze River, Guangdong, Inner Mongolia and other places, and the results is were very successful. Based on the data of Round-trip Drifting Sounding experiment in the middle and lower reaches of the Yangtze River from March to September 2021, this paper completed the verification of atmospheric humidity profile data of satellite. The results show that: 1)The validation results of the Descending Phase data of the Round-trip Drifting Sounding indicate that the average absolute error of the satellite humidity profile is approximately 15%, and the root mean square error is approximately 20%. 2) At noon and night, the quality of humidity profile data of satellite is better than that in the morning and evening. 3) The humidity error of the satellite decreases with increasing altitude and increases with increasing humidity. 4) Below 50% humidity, the satellite retrieved humidity is relatively large, and above 50% humidity, the satellite retrieved humidity is relatively small.